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WebAfter blocking with 3% H 2 O 2, the sections were incubated overnight with primary antibodies (EIF4G1, CST, 8701S, rabbit; mTOR, CST, 5536T, rabbit) at 4°C. Following three washes with phosphate-buffered saline (PBS) for 15 minutes each, the sections were incubated with a secondary antibody (mouse anti-rabbit IgG-HRP, Santa Cruz, sc-2357) …
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